In Vampire the Masquerade Bloodlines, female player-characters can flirt with, seduce, and in one instance have sex with female non-player characters (with the exception of characters in Giovanni’s Mansion– see here and here). For instance, male and female player-characters can have sex with the non-player character Jeanette, provided certain in-game conditions are met. The sex is only implied through voice-acting over a black screen, however. A video of this scene can be viewed here. Players can also hire sex workers who they kiss and then drink the blood of, as seen in this video.

Male PCs cannot, for the most part, flirt with male NPCs, and there are no male same-sex sex options. There are official game patches that re-enable the bisexuality of two male NPCs (the clerk at the Empire Hotel and Phil, according to this forum thread). There is also reportedly a mod that makes the character Romero bisexual (though none seem to be available any longer). Players have complained that this means there are generally more flirtation options for female PCs (here, here, and here).

LGBTQ  references in this game: 

Pisha

Same-sex Flirtations

Citations

1. A couple of questions. (n.d.). Retrieved from http://www.gamefaqs.com/boards/914819-vampire-the-masquerade-bloodlines/49860857?page=1
2. Blooddoll Bois. (n.d.). Retrieved from http://www.gamefaqs.com/boards/914819-vampire-the-masquerade-bloodlines/49334227
3. Fun with Pestilence. (n.d.). Retrieved from http://www.gamebanshee.com/vampirebloodlines/walkthrough/funwithpestilence.php
4. Gay Option. (n.d.). Retrieved from http://tvtropes.org/pmwiki/pmwiki.php/Main/GayOption
5. Gender. (n.d.). Retrieved from http://www.gamefaqs.com/boards/914819-vampire-the-masquerade-bloodlines/45819856
6. Jeannette. (n.d.). Retrieved from http://whitewolf.wikia.com/wiki/Jeanette_Voerman
7. kronx1999. (2008, November 5). vampire bloodlines lesbian hooker at a club and a guy pissing. YouTube. https://www.youtube.com/watch?v=8A4nY_s7w3o
8. Lesbians. (n.d.). Retrieved from http://www.gamefaqs.com/boards/914819-vampire-the-masquerade-bloodlines/55669732
9. Lesbian Vampire. (n.d.). Retrieved from http://tvtropes.org/pmwiki/pmwiki.php/Main/LesbianVampire
10. LGBT characters in video games. (n.d.). Retrieved from http://en.wikipedia.org/wiki/LGBT_characters_in_video_games
11. Main Vampire the Masquerade: Bloodlines Discussion. (n.d.). Retrieved from http://tvtropes.org/pmwiki/remarks.php?trope=Main.VampireTheMasqueradeBloodlines
12. Male Blooddoll in Clinic. (n.d.). Retrieved from https://forums.planetvampire.com/index.php?PHPSESSID=f3f2vnb9tre0aorrlcbpm1hch3&topic=5557.0;wap2
13. Queering Vampire: The Masquerade. (2012, June 24). Retrieved from https://leonhart.dreamwidth.org/16540.html
14. SephirothJosh48. (2014, September 6). Vampire Masquerade Bloodlines, lesbian lovemaking with Jeanette. YouTube. https://www.youtube.com/watch?v=d5-VDGfhZuc
15. So, um, how does Kindred sex work exactly? (2014, April 29). Retrieved from http://www.gamefaqs.com/boards/914819-vampire-the-masquerade-bloodlines/50389280
16. Where do I top up my blood. (n.d.). Retrieved from http://www.gamefaqs.com/boards/914819-vampire-the-masquerade-bloodlines/47230923
17. You can have sex? (n.d.). Retrieved from http://www.gamefaqs.com/boards/914819-vampire-the-masquerade-bloodlines/50389280
18. Zahn, J. (n.d.). Bloodlines Jeanette/Tourette conversation FAQ. Retrieved from http://www.gamesradar.com/cheats/14292/