Nina is one of the 2nd-generation child units the player can recruit to their army once her father, Niles, has an S-rank with another female unit in your army. As a character, she is known for her obsession with men being more than friends (as shown below with support dialogue text). Because of this, many fans have associated her with loving yaoi (Japanese boy-on-boy love).
Corrin: No, of course not. But now I’m curious… What have you been up to, Nina?
Nina: Oh, nothing interesting at all. Nothing even worth talking about. I suppose if I had to say SOMETHING, I would say I was…people watching.
Corrin: People watching?
Nina: Yeah! Like, see that guy over there? The one dripping with sweat from practice?
Corrin: Yeah, looks like he had quite a workout.
Nina: And then over there, there’s a guy tending to some horses. What if those two guys met up…like, for a cup of tea? Wouldn’t that be splendid? What would they talk about? Would they become fast friends? Or something more…
Corrin: Something more? I’m not sure I understand.
Nina: Oh, well, never mind that, then! Just imagine a good, old-fashioned friendship. But then one day they find themselves in the middle of an intense staring contest… Hee hee!
Nina: All right, then. Let’s say—hypothetically, of course—that I love spying on men. And not just any men. Men in close relationships. Men who are best friends. Weirded out yet?
The second great human country, Ydor, is the kingdom of art. Much more open minded than its neighbor Sarona, you can see openly gay couples, statues of naked men, and drag queens.
There is a steam achievement when playing this game called, “This is my hair, I don’t wear wigs,” that you can unlock if you speak to the drag queen wearing the wig hat, which is a reference to an Alaska Thunderfuck song.
In the world 1 hub, if you play a special melody on the medusa, you can unlock a hidden level where you can meet a mermaid, a being that is a mix between a human and an octopus. She tells you that you will always meet people that don’t fit inside any box, that they are valid, and that her cave is a safe space.
The Warden Game is a text-adventure style game that helps players understand the contradictions of prison life in an age of mass incarceration. Designed by Ed Mead around 1987, while he was incarcerated at the Washington State Reformatory at Monroe, the game explores power in prison and how individuals exercise it. Being a queer prisoner himself, Mead included mentions of anti-sexist education, since in 1977, Mead cofounded “Men Against Sexism” at Walla Walla state prison. MAS used methods such as peer education and direct confrontation to stop the rape and abuse of gay and effeminate prisoners.
The Warden Game was adapted from Ed Mead’s original programming and developed for the web in 2017 by Magdalena Donea and Dan Berger.
Hifumi Yamada is one of the teens trapped inside Hope’s Peak Academy and forced to play the Killing Game. In School Mode, during Hifumi’s ending dialogue, Hifumi references yaoi when Makoto takes Hifumi’s comment about “holding onto him” as weird (found here).
Toko Fukawa is a teen trapped inside Hope’s Peak Academy and forced to play the Killing Game. Genocide Jack, a serial killer, is her other personality, as she has Dissociative Identity Disorder. Throughout the game, Toko and Genocide Jack both talk inappropriately about multiple characters, but in Chapter 2, Genocide Jack suggests that Hina and Sakura, two other teens trapped inside Hope’s Peak Academy and forced to play the Killing Game, are into “girl-on-girl action” (found here). In Chapter 4, Toko talks about how “disgusting” she thinks Hina is, leading to what seems like Toko thinking of Hina in a sexual way (found here). Both Toko and Genocide Jack seem to worship one of the male characters named Byakuya Togami as their “master” throughout the game as well, in addition to Genocide Jack only targeting “cute boys”, so it is unknown what Toko and Genocide Jack’s sexual orientations are.
Chihiro Fujisaki is one of the teens trapped inside Hope’s Peak Academy and forced to play the Killing Game. In the second chapter of the game, it is revealed that Chihiro is biologically male and dresses as a girl to deal with an inferiority complex. Chihiro was bullied as a child and told that he was weak for a man, resulting in him deciding to dress up as a girl as a way to be socially accepted as “weak”.
Many fans have speculated whether Chihiro is a trans character or not, but canonically, it seems that Chihiro identifies with the gender he was assigned at birth according to a conversation Chihiro has with Makoto during “School Mode” (found here) During the second chapter of the game, there are trans shock “she was a he?!” tropes played up as the rest of the cast find out about Chihiro’s secret (found here).